Part:BBa_K2725015:Design

J23103 - supD - B1006

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 77
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 80
  

Design Notes

J23103 Anderson promoter positioned same number of bases upstream of supD as pLac in BBa_K914000.

Source

supD sequence from BBa_K914000. Promoter sequence: BBa_J23103. Terminator sequence: BBa_B1006.